A. Label the X axis for 0%, 25%, 50%, 75%, and 100% dilutions.
B. Label the Y axis for appropriate levels based on mean values
or test assay range.
C. Plot coordinates. (0%, mean Pool 1), (25%, mean Mixture
2), (50%, mean Mixture 3), (75&, mean Mixture 4), (100%, mean
D. Draw best line through data points giving more weight to
the first 3 coordinates.
E. Inspect plot for linearity and make a visual decision as
to acceptable reportable range.
When the above points are plotted, the following graph results:
The linearity of this example is very good.